Toxicity of insecticide mixture is sum of its parts, a cell study finds.

Aug 10, 2011

Cao, Z, TJ Shafer, KM Crofton, C Gennings and TF Murray. 2011. Additivity of pyrethroid actions on sodium influx in cerebrocortical neurons in primary culture. Environmental Health Perspectives http://dx.doi.org/10.1289/ehp.1003394.

Synopsis by Tamara Tal

Widely used pyrethroid insecticides that share a common nerve cell target produce a larger toxic effect than each chemical on its own, according to a rodent brain cell study that verifies prior animal studies.

A mixture of almost a dozen, similar-acting insecticides that are used on food crops can act in concert to produce effects in mouse brain cells on par with the sum of each chemical's individual effect, reports a team of academic and government researchers. Together, the 11 pyrethroid insecticides – tested at levels that mimic real-world exposures – over-stimulate electronic channels in the cells, an action that eventually kills them. 

This study is important because it shows for the first time that common insecticides converge on the same cell target to produce heightened toxicity. Scientists call this type of increased toxicity “additive" because the mixture produces a toxic response that is equal to the sum of each individual effect.

Pyrethroids are synthetic insecticides found in more than 3,500 registered products, many of which are used in homes, on pets and in agriculture. These insecticides are designed to mimic the effects of the natural toxins found in chrysanthemum flowers.

Pyrethroid insecticides are toxic to insect pests and humans in the same way. They target vital electrical channels – called voltage-gated sodium channels – in certain types of brain cells known as neurons. The chemicals essentially boost the electrical signals that are moving into the cells. The over-activation of these channels changes neuron function and ultimately leads to paralysis and death in the targeted pest.

Researchers at Creighton University and the U.S. Environmental Protection Agency collected mice brain cells and exposed them to the pyrethroids singly or in a mixture. They wanted to test whether the combined effect of multiple pyrethroids is equal to the sum of their individual effects. They used nerve cells from mice to understand what might happen in humans.

The researchers found differences in each chemical’s ability to affect the cellular channels. Seven triggered a strong effect, two pyrethroids produced a mild response, and two had no effect. A known inhibitor blocked the actions of the active pyrethroids. Together, these confirm that the active chemicals have the same target.

When the researchers exposed neurons to all 11 compounds simultaneously, they found that the combined effect of the mixture was equal to the sum of the effects of the individual pyrethroids.

This study is important because it confirms the prevailing assumption that pyrethroid mixtures produce additive toxicity by converging on electrical channels in brain cells.

The pyrethroid compounds tested – deltamethrin, β-cyfluthrin, cypermethrin, permethrin, bifenthrin, esfenvalerate, λ-cyhalothrin, tefluthrin, fenpropathrin, resmethrin and S-bioallethrin – are mainly used to control pests on food crops.

 

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